To focus of an object, start on the scanning objective first, focusing using the coarse adjustment knob and the fine adjustment knob as needed. Then, switch to the low power objective using the coarse adjustment and fine adjustment knob as needed. Finally, make sure the coarse adjustment knob is lowered before switching to the high power objective because the lens could be scratched. Only use the fine adjustment knob to focus on the object.
To make a wet mount on a slide, take your specimen and place it in the middle of your slide. Make sure that it isn't too thick or else it will make it harder to observe it. Next, place one drop of water over the specimen. Then, place the cover slip at a 45 degree angle with one edge touching the water drop and gently let go.
To stain a specimen, follow the same steps to make a wet mount. Next, place one drop of methylene blue on the edge of the cover slip. Then, place the edge of a paper towel on the opposite side of the cover slip. The paper towel will draw the water out from under the cover slip and pull the dye under the cover slip.
Doing this lab didn't really affect my microscope skills at all. I learned all this information when I was in middle school. The only thing I can think of is that you only clean the lenses with lens paper. Other than that, I knew all the information.
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